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For retrograde tracing from the small intestine to the DMV, FG (fluorochrome 4% in PBS, 10 L) was injected into the small intestine, or scAAV2/Retro-hSyn-Cre-WPRE (20 L) was injected into the small intestinal wall at different sites (200 nL per site) and AAV-DIO-mCherry was injected into the DMV of C57 mice

37 However, these studies were not primarily designed to assess renal outcomes and the benefits seen were mainly attributable to reductions in albuminuria

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[21] reported an alertness-increasing effect of VitB12 supplementation, with a decrease in sleep duration

This was done for both T51B and RL-34 cell lines

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