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in Assay Guidance Manual (eds Sittampalam, G

All compounds were dissolved in dimethyl sulfoxide (DMSO) and diluted with sterilized distilled water until the concentration of DMSO was 1% for 30 min in a 50 l reaction mixture containing 50 mM Tris-Cl (pH 8.0) and 5 mM DTT

Although low levels of reactive oxygen species (ROS) mediate critical physiological signaling pathways, accumulation of raised levels, if not cleared rapidly, can lead to major deleterious consequences affecting several organs, such as the heart, kidney, eye and brain, culminating in a variety of human disorders (Forman and Zhang 2021)

When administered prior to injury, the peptide appeared to reduce damage severity in controlled settings

The remaining 13 subjects successfully completed the protocol

Last dose was last evening