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McCoy and colleagues reported that 5-day treatment of dissociated rat hippocampal neurons with Dihexa increased dendritic spine density approximately three-fold compared to vehicle control (~41 spines per 50 m of dendrite versus ~15 spines), with the increase reflecting both new spine formation and increased spine-head width consistent with synaptic maturation [2]

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Together, these data demonstrate that mutations in ECLs can specifically affect the activation of GLP-1R TMD by EX4-ECD when provided in trans, suggesting that these mutated residues could serve as the contact sites between ECD and TMD

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