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A poor diet, insufficient exercise, and the consequent weight gain are the primary causes of the condition in most instances (14)

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Journal Of The American College Of Cardiology 2016, 67: 1027-1035

The gene fragments were cloned into with BamHI (NEB, Frankfurt, Germany) digested and dephosphorylated (Antarctic phosphatase, New England Biolabs, Frankfurt, Germany) expression vectors by Gibson-Assembly