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While the IOM had based B12 needs on the amount required to correct hematological parameters for people with pernicious anemia, the EFSA had almost 20 more years of research to draw on, and concluded that the most suitable approach was basing needs on biomarkers (serum B12, holotranscobalamin, MMA, and homocysteine)
After centrifugation at 1300 g for 15 min, the supernatant was collected by filtration through a 0.45 m filtration membrane
However, taking them together isnt likely to bring any negative side effects
