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Khorsandi K, Fekrazad S, Vahdatinia F, Farmany A, Fekrazad R
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Pellets were thawed on ice for 1 h and lysed in 200 l 1% SDS in PBS supplemented with 1 protease inhibitors (cOmplete, EDTA-free Protease Inhibitor Cocktail, Roche, 11836153001) and shaken for 30 min at room temperature with 1:1,000 v/v benzonase nuclease (Sigma, E1014)
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Research designs targeting this tissue type typically rely on imaging methods capable of distinguishing organ-adjacent fat from fat stored elsewhere in the body
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