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When cells had reached a confluence of 60%, glass coverslips were washed twice with PBS and cells were fixed in 4% PFA for 10 minutes

Looking Deeper One of the primary causes of the DNA damage that leads to skin cancer is exposure to ultraviolet (UV) radiation from the sun and tanning lamps

That works but is a larger injection

The solution is simple: store vials in amber glass (which filters UV and short-wavelength visible light) or wrap clear vials in aluminium foil or opaque bags

Medeiros JL, Costa WS, Felix-Patricio B, Sampaio FJB, Cardoso LEM

Her body was asking for less and healthier food, but she did not diet or exercise