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Founder pups were genotyped as described below and backcrossed to C57BL/6J mice for at least four generations before experimentation

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Here is what the data shows for each major option

We noted that SC-islets exhibited both lower oxygen consumption following oligomycin exposure and a higher peak oxygen consumption after the uncoupler FCCP compared to human islets, which had not been observed previously

Blood 130:22372237 Akhter MS, Hamali HA, Rashid H, Dobie G, Madkhali AM, Mobarki AA, Oldenburg J, Biswas A (2023) Mitochondria: emerging consequential in sickle cell disease