Acylcarnitine assay Acylcarnitines were extracted from either 10 l of whole liver homogenate, 50 l of plasma, 50 l brain parenchyma, 50 l cerebrovasculature (timepoint study), 60 l of cerebrovasculature (cerebrovascular uptake study) or 100 l of brain homogenate (all homogenized as described above) and spiked with 5 l of acylcarnitine internal standard (IS) mix containing 12 M TMAO-d9 (Cambridge Isotope Laboratories, Inc., CIL), NSK-B (CIL) and NSK-B-G1 (CIL) at the beginning of the extraction process
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Oxidative Stress: Placenta Function and Dysfunction
Liu, W.J
reuteri possesses the capability to impact the host behavior through vagus nerve 58
After centrifugation, the pellet was resuspended in red cell lysis buffer (150 mM NaH 4 Cl, 10 mM NaHCO 3 , 1.27 mM EDTA) and incubated for 2 min at RT with gentle manual agitation and centrifuged again
