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Synthetase reactions (200 L) contained 5 L of homogenate with 10 M 14 C-fatty acid in a buffer of 40 mM TrisHCl, 5 mM ATP, 5 mM MgCl 2 , 4 mM CoA, 0.8 mg/mL Triton WR1339, and 1 unit/mL inorganic pyrophosphatase

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