Further, the SOE PCR products were cloned into FastDigest BshTI/FastDigest BglII (Thermo Fisher) linearized pEGFP-C2 vector using ClonExpress II One Step Cloning Kit (C112, Vazyme) to obtain the six sorting vectors, pEGFP-GST-GPI DAF , pEGFP-GST-GPI BY55 , pEGFP-GST-GPI CEAM7 , pEGFP-GST-TMD ITB3 , pEGFP-GST-TMD ITA5, and pEGFP-GST-TMD ITAV
Se aconseja de 4-6 sesiones cada 15-21 das.
[DOI] [PubMed] [Google Scholar] 104.De Crescenzo F, DAl GL, Morgano GP, Minozzi S, Mitrova Z, Saulle R, et al
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