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After 48 h, DMEM was replaced with Neurobasal medium (Gibco, 21103049) containing 50 B27 (Gibco, 17504044), 100 glutamine (Invitrogen, 25030081), and 100 penicillin-streptomycin (Thermo, 15140122)

Further decomposition analysis of the data reveals that the energetic contribution of 13 important residue pairs is conserved between the SR18GLP-1R and GLP-1GLP-1R complexes

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