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DiMarchi had just left Lilly after working there for 22 years where he and Tschp had first met

After subsequent washes (to allow any unbound FAM-FLICA to diffuse out of cells), nuclei were stained with Hoechst 33,342 (1 g/ml) for 10 min at 37 C and cells were examined under a Zeiss Axiophot fluorescence microscope using a 40 /1.3 N.A