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2.11 Separation and extraction of nuclear and cytoplasmic proteins In this experiment, nuclear and cytoplasmic fractionation was performed using the Nuclear and Cytoplasmic Protein Extraction Kit (Beyotime, P0027) following the manufacturers instructions

Given that obesity in adolescence is already associated with an increased risk of depression and suicidal ideation, and considering the rise in GLP-1 receptor agonist prescriptions since FDA approval in 2020, it was crucial to investigate whether this treatment impacts psychiatric outcomes

Plants grown in antioxidant-treated regolith simulant for 59 days displayed an increased biomass of 1.72 gm on average compared to untreated regolith plants (ave = 0.02 gm, p = 0.0337 calculated as one-way ANOVA) ( Figures 1C, D )

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They typically present as small, dry, red and rough patches of skin on the scalp, face, hands and lower legs (see Figure 1)