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For example, we would not have timely EBCM data available for the new TIN/NPI combination to determine if the ASM participant meets the 20 EBCM episode volume criterion (discussed in section III.C.2.c.(3)(b) of this final rule) under the new TIN

The proteins on the PVDF membrane were blocked with 5% skim milk (1 h at room temperature), followed by an overnight incubation (4 C) with the primary antibody

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