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After putting the finished reaction mixture in a heat cycler, the following procedure was run: primer annealing for 10 min at 25 C, reverse transcription for 15 min at 42 C, and inactivation for 5 min at 85 C

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Additionally, if tirzepatide enables corticosteroid dose reduction, the bone-protective effects of lower steroid exposure could outweigh any bone density loss from weight reduction

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