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Expression of a lysosomal-targeted NNT homolog would likely increase the cytoplasmic [NAD + ]/[NADH] more effectively than increasing the cytoplasmic [NADPH]/[NADP + ] due to the limiting 0.1 M cytoplasmic [NADH] that is approximately 1080-fold lower than the cytoplasmic [NADP + ]

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Gupta MP

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This step is particularly important for peptides used in in vitro cell assays