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Each sample was homogenized using a TissueLyser III (Qiagen) with 5 homogenization beads in 200 l of cold Tris lysis buffer (150 mM NaCl, 20 mM Tris, pH 7.4, 1 mM EDTA, 1 mM EGTA, 1% Triton X) containing a cocktail of protease (cOmplete Protease Inhibitor Cocktail, Roche) and phosphatase inhibitors (Phosphatase Inhibitor Cocktail 2 and 3, Sigma Aldrich), NaF and PMSF in DMSO

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The morphology of FLS was assessed by an inverted microscope with a Leica Qwin System (Leica, Germany)

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Saxenda Le Saxenda (liraglutide) est un mdicament base de GLP-1, utilis dans le traitement de lobsit et du surpoids avec facteurs de risque