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Protein shakes are a practical option here, particularly whey or casein protein, which have the most evidence for supporting muscle protein synthesis

RNA isolation and qRT-PCR Total RNA was extracted from primary astrocytes and striatum tissue using TRIzol reagent (Invitrogen, 15596026) and reversed transcribed into cDNA using PrimeScript RT Master Mix (Takara, RR036A)

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Under this anesthesia a lot of the autonomic reflex functions are studied and are operational [24, 26, 29, 31,32,33] and therefore being used in current study examining effects of GLP-1 on neural circuitry of renal nerve activity involved in central nervous system