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After washing steps, DNA was eluted twice with elution buffer (25 mM TrisHCl pH 7.5, 5 mM EDTA pH 8.0, 0.5% SDS, 65 C, 20 min), treated with 10 mg/mL RNase-A (65 C, 1 h) and with 20 mg/mL proteinase K (65 C, 5 h), then was purified using the AxyPrep DNA Purification Kit (Axygen, AP-GX-250)
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Quality Matters: Why Your Peptide Source is Everything We've spent this entire article discussing procedure and technique, but all the perfect technique in the world can't fix a low-purity product
doi: 10.1083/jcb.201102031 36 CorreiaMPinheiroPBatistaRSoaresPSobrinhoSimesMMximoV
Zinc and the Msc2 zinc transporter protein are required for endoplasmic reticulum function