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Briefly, 10 l of serum was incubated with the top 12 abundant serum protein depletion kit (Thermo Scientific Pierce, Cat# 85164) and digested with trypsin on S-Trap column (ProtiFi, NY)

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D., Liu, H., Lacorre, D

Reconstitute the peptide with Bacteriostatic Water before use, refrigerate the solution, and use it promptly to achieve consistent experimental results

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