glp 1 gip gcg Effects of site-directed mutagenesis of GLP-1 and glucagon receptors on signal transduction activated by dual and triple agonists The activity of GLP-1 and

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Genomic DNA was then isolated with the help of NucleoSpin Tissue XS from the clonal cell lines and subjected to sequencing to identify frameshift mutations by amplifying the genomic regions with the following primers: for ARL8A KO, forward 5- GATTCCGGGGCAGCGAGTCGT-3 and reverse 5-TACTAGGCCCCAAGCGCTCG-3, and for ARL8B KO, forward 5-TCATCTCCCGCCTGCTGGACT-3 and reverse 5-TCCAAATGCTGGCCCTCGG-3