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The off-cycle period allows the body natural repair mechanisms to operate independently and prevents dependence on exogenous peptide signaling
To process the spleens, tissue was placed in a 35-mm petri dish in 10 mL PBS-FBS and ground between frosted glass slides (Thermo 2955) before filtration to generate a single cell suspension
Modifier LT identifies a left-sided unilateral injection
Sometimes it delivers nothing at all
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These timepoints were selected to represent distinct stages of iHLO development and maturation: Day 7 signifies the hepatoblast differentiation stage, Day 15 marks the early hepatocyte stage, and Day 30 and Day 45 indicate progressively matured liver organoid stages with increasing liver cell populations and functions
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