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Throughout the experimental period, the water temperature ranged from 26 to 29 C, dissolved oxygen was maintained between 5.0 and 7.5 mg/L, and pH levels ranged from 7.2 to 7.8, the concentration of ammonia nitrogen was below 0.02 mg/L

Week 4: 7.5 mg = 0.75 mL

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7f), suggesting that HNF4-regulated cysteine metabolism significantly contributes to cellular sensitivity to MCR

For instance, HMGB1 can regulate calcium levels through a TLR4/ROS-dependent pathway, leading to calcium leakage from the sarcoplasmic reticulum, a reduction in the amplitude of calcium transients, and decreased cardiomyocyte contractility, which can further aggravate the pathological processes in the heart [307]