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As a template for real-time PCR, 0.39 L of cDNA was amplified in 5 L final volume

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Research using LPS-stimulated macrophage models and UV-challenged skin cell lines has documented percentage reductions in IL-6 and TNF-alpha secretion following GHK-Cu treatment, with some studies reporting reductions in the 30 to 60% range compared to untreated challenge controls