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The authors also thank Tine Aggerholm Bkdal and Ayna Baladi Nejad, former employees of Novo Nordisk A/S, for their contributions to both studies

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The isoelectric point of a nucleotide is identified as a specific pH value where the amount of positive and negative charges within the nucleotide equate, yielding a net charge of zero. Phosphate groups constitute a crucial component of nucleotides

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The CHARMM-GUI website ( was utilized to select the CHARMM36 force field for generating system input files 77,78

170 Sarchielli, P., Coata, G., Firenze, C., Morucci, P., Abbritti, G