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When a drug gets FDA approval, it means its been rigorously tested for a specific use (this is called on-label)

Takes 510 minutes

It comes down to what your protocol actually requires

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One freeze-thaw cycle may cause minimal loss

The Mayo Clinic indicates high doses of vitamin B-12, such as those used to treat a deficiency, might cause: Headache Nausea and vomiting Diarrhea Fatigue or weakness Tingling sensation in hands and feet Healthline says: In very rare cases, some people can experience side effects caused by allergic reactions or sensitivity In addition to helping athletes build muscle, vitamin B12 plays a role in maintaining nervous system function and brain health