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MTT Cell Proliferation and Cytotoxicity Assay Kit (batch number C0009S) was obtained from Beyotime

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Maeda N, Shimomura I, Kishida K, Nishizawa H, Matsuda M, Nagaretani H, et al

As illustrated in Additional file 1: Figure S1C, animals from both TAT and TAT-DAT NT did not differ in time spent in each duration (two-way ANOVA with repeated measures, F 1, 10 = 0.321, p = NS), suggesting that the differences in spontaneous alternation behavior were unlikely due to confounding factors such as anxiety

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Dietary contribution is approximately 300 mg/day with a total pool size of 1200 mg in men and 600 mg in women [10, 11]