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This was because it challenged the prevailing assumption about the biological mechanism of glucagon

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If not, the absorption rate is likely very low

Co-cultures were stimulated with 50 ng/mL phorbol-12-myristate-13-acetate (PMA) and 750 ng/mL ionomycin (both Sigma-Aldrich) for 4 h in the presence of 2 M monensin (Biolegend) at 37 C and 5% CO 2 /95% humidified air