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The most widely used detection method involves the derivatization with 2,4-dinitrophenylhydrazine (DNPH) and spectrophotometric quantification at 370375 nm, with the absorbance being proportional to carbonyl content
10.1530/REP-20-0037 39 XuK.TianY.WengX.HuX.HengD.XiaG.et al (2020)
Upon interaction with GSH, IR806-PDA is converted into IR806-S-NH, resulting in a spectral shift (from 680 to 820 nm) and generating ratiometric photoacoustic signals that correlate with GSH levels