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In activated T cells, metabolic fitness and synthetic function has been directly linked to G6PD activity and ROS homeostasis by Ghergurovich and colleagues, who used a novel metabolite reporter and deuterium tracer assays to monitor cellular G6PD activity in the presence of a specific potent inhibitor of G6PD (55)

siRNA or siNC (5 l) and transfection reagent GP-transfect-Mate (4 l) were added into 300 l OPTI-MEM (Gibco

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