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-galactosidase activity was detected using X-Gal staining solution (1 mg ml 1 X-gal, Thermo Scientific, 5 mM K 3 (Fe(CN) 6 ) and 5 mM K 4 (Fe(CN) 6 )) diluted in 1 mM MgCl 2 /PBS (pH 5.56) for 818 h at 37 C

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Given that FXS is a monogenic cause of ASD, these findings highlight both the challenges of translating GABAergic modulation into broad clinical efficacy and the importance of patient stratification in future trials of targeted therapies for neurodevelopmental disorders (152)

Samples were placed briefly on a TuboVap (Zymark, Massachusetts, US) to remove the organic solvent