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Tissues were lysed by transferring them to pre-filled bead mill tubes (Fisher Scientific, 15-340-154) containing lysis buffer (Tris-HCl 100 mM and 0.5% Triton X-100, pH 8) containing Halt Protease and Phosphatase Inhibitor cocktail and homogenized using a bead mill (VWR) for 10 s
Each syringe type is marked at a different number of units per mL: U-100 reads 100 units per mL, U-50 reads 50 units per mL, and U-40 reads 40 units per mL
Ingredients: Concentrated apple cider vinegar, dried pomegranate juice, dried beetroot juice, Vitamin B12, folic acid, natural flavor
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