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these inhibitors include aurora-A, ZNF498, and eupaformosanin (Hsueh et al., 2013

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After centrifugation at 30,000 g for 30 min, the supernatant fraction was loaded onto a nickelimmobilized metal ion affinity chromatography (NiIMAC) column (His GraviTrap, GE Healthcare)

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They found that the 2D motility of HT-1080 fibrosarcoma cells, MDA-MB-231 breast cancer cells, and normal breast epithelial MCF-10 A cells is faster on fast- (~ 100 s) relative to slow-relaxing (~ 2,000 s), soft (2 kPa) substrates [143]

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