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Both cells were cultured at 37C with 5% CO 2 , generally subcultured every 23 days, and regularly tested for mycoplasma using mycoblue mycoplasma Detector (D101-02, Vazyme)

An increase of LC3 puncta does not necessarily indicate autophagy enhancement since downstream suppression of autophagy could also instigate a similar effect

Once all 3 mL of water has been added, slowly withdraw the needle

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This hormone plays a key role in regulating blood sugar, appetite, and even some aspects of inflammation