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For pdbqt preparation, the prepare_ligand4.py script from AutoDockTools 1.5.7 (Trott & Olson, 2010) was employed, retaining all atoms without cleanup (-U) while repairing hydrogens (-A) to ensure compatibility with docking calculations

Membranes were probed with anti-GCLC sc-390811 (200 ng/ml), anti-ACTB sc-69879 (5 ng/ml), and anti-Vinculin sc-73614 (200 ng/ml), both from Santa Cruz Biotechonology (Santa Cruz, CA, USA) followed by goat anti-mouse IgG conjugated to IR-800Dye (926-32210

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Milk thistle may be useful in treating liver disease, cancer, and diabetes