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Post column-switching, analytes are separated and determined using a guard column (Dionex IonPac SGS, 50 mm 4 mm, i.d.) and an analytical column (Dionex IonPac SCS, 250 mm 4 mm, i.d.) with an isocratic mobile phase comprising 3 mM MSA and 10 % (v/v) acetonitrile, at a flow rate of 1 mL/min

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Nat Rev Neurol 19:715736