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Protein-ligand interactions were characterized using the PLIP server, visualized in three dimensions with PyMOL, and the stability and reliability of the best docking pose were validated through molecular dynamics simulations

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The analysis typically considers four factors: the physical and chemical characterization of the substance, any safety issues raised by using the substance in compounding, the available evidence of effectiveness for the use, and historical use of the substance in compounding, including the medical condition being treated and any references in the medical literature