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Erythrocytes were lysed twice by adding DEPC-treated H 2 O and NaCl (8.8%), followed by centrifugation (450 x g, 6 min, 4C, Universal 320 R, Hettich, Tuttlingen, Germany)

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For copper peptide research specifically, this study demonstrates that delivering copper peptides directly to the follicle (bypassing the skin barrier through microneedling) produces significantly stronger results than topical application alone

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[DOI] [PMC free article] [PubMed] [Google Scholar] Ofir R, Dwarki VJ, et al