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Conclusion: Despite a low burden of established CVD, young ESUS patients harbored pathology in the vascular aging measures

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Eur J Drug Metab Pharmacokinet 41:839844

Dissociated cells were grown in Neurobasal medium (ThermoFisher, 21103049) supplemented with 2.5% ( v / v ) B27 supplement (ThermoFisher, 17504001), 0.5 mM L -glutamine (Sigma-Aldrich, G7513) and 5 g/ml plasmocinTM (InvivoGen, ant-mpt), and plated onto poly- D -lysine (Sigma-Aldrich, P6407)- and laminin (Sigma-Aldrich, L2020)-coated plates at a density of 2 10 5 cells/cm 2

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As you get older, the structures in your spine begin breaking down and increase your risk for spinal narrowing