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We also performed the experiment in the non-fasting condition

This is why many researchers reconstitute only what they need for the near term and keep remaining powder in long-term storage

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The caloric composition of the normal chow diet consisted of 23% protein, 21.6% fat, and 55.4% carbohydrates, while the HFD contained 20% protein, 60% fat, and 20% carbohydrates