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A 20 l mix, containing 8 l of cDNA sample, 10 l of SYBR Green PCR Master Mix (Thermo Fisher Scientific), and 2 l of 10 mM forward primer and reverse primer mixture was used to performed Quantitative RT-PCR with a StepOne Plus Real-time PCR system (Applied Biosystems, Foster City, CA, United States)

Reconstituted solutions should be used promptly or stored at 2-8C and used within 60 days, as reduced glutathione is susceptible to oxidation

Logistics: Requires sterile supplies (alcohol wipes, insulin pins) and proper sharps disposal

A brief warm sensation or flushing can also occur

Preserves mitochondrial function in gametes

Prudent approaches include: Cycling: Periodic breaks from peptide use