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Major Depressive Disorder (MDD) specifically has an alarming presence across age groups, with a 12-month prevalence of 10.4% and a lifetime prevalence of 20.6% [487]
Cytochrome c release assay Cells were fractionated to obtain cytoplasmic and mitochondria-enriched fractions using Dounce homogenization and differential centrifugation as per the Abcam cytochrome c release kit (ab65311)
Disruption of the gut microbiota early in life, for instance via antibiotic exposure, can result in long-lasting changes to visceral pain sensitivity and stress responsiveness, as demonstrated in rodent models (O'Mahony et al., 2014
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This protocol uses a 3.0 mL dilution to maintain accurate and consistent dosing per injection
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