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Compliance with ethics guidelines Approval for this study was obtained from the Human Research Ethics Committee of the Medical University of Lodz, Poland (RNN/2019/18/KE)

Anthropometrical and clinical parameters were systolic and diastolic blood pressure, heart rate, BW, waist circumference (WC), and body mass index (BMI)

( i ) Michaelis-Menten plot for in vitro cleavage kinetics with native substrate (top) and kinetic parameters (bottom) for BoNT/X protease variants

The liver damage from paracetamol poisoning may not be visible for 2472 hours, but an antidote called N-acetylcysteine is most effective when given early ideally within the first few hours

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Next, 25 g protein from each sample was reduced with 2 mM dithiothreitol, followed by incubation at 55 C for 60 min