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Contraindications include personal/family history of medullary thyroid cancer, multiple endocrine neoplasia type 2, pancreatitis, and severe gallbladder disease

RNAscope in situ hybridization was performed on these sections using the RNAscope Multiplex Fluorescent Kit (v.2) (Advanced Cell Diagnostics) as per the manufacturers instructions, with a modification to the pre-treatment procedure (Protease IV incubation conducted for 20 min at room temperature)

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( B ) Reduction of cyt c (20 M) over time, in the presence of GSH (1 mM), plus/minus ascorbate (Asc, 0.5 mM), plus/minus S-nitrosoglutathione (GSNO, 1 mM), measured by absorbance at 550 nm

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