The mice were housed in groups of three animals during the study, on a normal 12 h light cycle (at 8:00 pm lights off), 22 2 C and 50 10% relative humidity
Pain perception is highly individual and influenced by multiple factors including needle phobia, injection technique, anatomical site selection, and previous experience with injectable medications
Apart from that, GLP-1Rs may directly affect neurotransmitter activity, which could contribute to improvements in mood and emotional well-being
It may increase the risk of low blood sugar, especially if consumed in excess or without food
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As GLP-1R mRNA in the aortic tissue was below the level of quantification in both animal models, it seems likely that GLP-1Rs in other tissues, rather than direct action of liraglutide or semaglutide on the vascular bed, contribute to the mechanism of gene regulation
