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If necessary, the eluted protein can be concentrated using ultrafiltration or dialysis against an appropriate buffer to remove excess glutathione and prepare the protein for downstream applications

10.1016/j.mehy.2012.02.023 Med Hypotheses

Frequency depends on personal goals (e.g., skin brightening, detoxification), lifestyle factors (e.g., stress, diet), chronic conditions, and your individual response to the therapy