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doi: 10.1158/1541-7786.MCR-20-0839 106 DamskyWECurleyDPSanthanakrishnanMRosenbaumLEPlattJTGould RothbergBEet al

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Qualitative assessment of nitric oxide production by fluorescent dye 3-Amino-4-aminomethyl-2,7-difluorescein, diacetate (DAF-FM DA, Sigma) HUVECs grown in M199 based culture medium was stimulated with 1.0 g/ml BPC 157 for 30 min to induce the nitric oxide generation