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The culture medium was replaced at each time-point and the cells were then incubated with 110 l complete medium containing 10 l Cell Counting Kit (CCK)-8 reagent (Beijing Solarbio Science & Technology Co., Ltd.) for 90 min at 37C

Cumbee SR, Pryor RE, and Linzer M

Contemp Oncol (Pozn) (2013) 17(1):15

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Subsequently, adapter trimming was performed using Cutadapt (v1.14), and low-quality reads were removed with Trimmomatic (v0.36)

We also required at 180.50(b)(2)(ii)(C) that hospitals calculate and encode an estimated allowed amount, in dollars, when hospitals have established a payer-specific negotiated charge that is based on a percentage or an algorithm