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Nuclear receptor coactivator 4 (NCOA4)-mediated ferritinophagy promotes ferroptosis in skin fibroblasts induced by oxygen and glucose deprivation, leading to delayed healing of ischemic wounds [77]
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After incubation with hypo-osmotic swelling solution [HOS, containing 6.75 g/L fructose and 3.67 g/L sodium citrate] at 37C for 1 h, plasma membrane integrity of each group (repeated in triplicated)was analyzed by evaluating the modifications of sperm tail to score swollen sperm according to previous publications (61)
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